CRISPR-powered optothermal nanotweezers
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Introduction
CRISPR-powered optothermal nanotweezers.The field of nanotechnology has revolutionized various aspects of our lives, from medicine to electronics. However, the manipulation and characterization of objects at the nanoscale remains a significant challenge. Conventional optical tweezers, while powerful, are limited in their ability to capture and manipulate a wide variety of nanoparticles. Furthermore, these tweezers lack the ability to identify the molecular composition of the trapped particles.
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To address these limitations, the researchers
developed a CRISPR-powered optothermal nanotweezer (CRONT). This innovative
technology combines the capabilities of optothermal manipulation with
CRISPR-based biodetection. CRONT uses a laser beam to generate a localized
temperature field that traps nanoparticles through diffusiophoresis and
thermo-osmotic flows. The CRISPR system, a revolutionary tool for gene editing,
is then used to identify the molecular makeup of the captured particles.
Principle of operation
This temperature gradient induces two physical
phenomena: diffusiophoresis and thermoosmotic flow. Diffusionphoresis is the
movement of particles from an area of low concentration to an area of high
concentration. In the case of CRONT, nanoparticles are attracted towards the
heated region due to temperature-induced changes in solvent density.
Thermoosmotic flow, on the other hand, is the movement of fluid driven by
temperature gradients. In CRONT, this flux helps trap nanoparticles near the
heated region.
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Once the nanoparticles are captured, the CRISPR
system is used to identify their molecular makeup. CRISPR, which stands for
Clustered Regularly Interspaced Short Palindromic Repeats, is a naturally
occurring bacterial immune system that has been reengineered as a gene-editing
tool. In CRONT, CRISPR is used to detect specific DNA sequences attached to
captured nanoparticles.
Wider range of nanoparticle capture: CRONT can
capture a wider range of nanoparticles, including DNA, proteins and viruses,
thanks to the optothermal capture mechanism.
Molecular identification: CRONT can identify the
molecular composition of captured nanoparticles using the CRISPR system,
providing valuable information for biological studies.
High sensitivity: CRONT can detect single nucleotide
polymorphisms (SNPs), making it a powerful tool for genetic analysis.
CRONT application
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Biomedical Research: CRONT can be used to study
individual biomolecules such as DNA and proteins at the single molecule level.
This can help to understand the basis of biological processes and develop new
diagnostic and therapeutic strategies.
Environmental Monitoring: CRONT can be used to
detect and monitor environmental pollutants and pathogens at extremely low concentrations.
Nanophotonics: CRONT can be used to manipulate and
study the optical properties of nanoparticles, which has applications in the
development of new optical devices and sensors.
Future instructions
Development of more efficient and versatile CRONT
systems
Expanding the range of biomolecules that can be
detected with CRONT
Integrating CRONT into microfluidic devices for
high-throughput analysis
CRISPR-powered optothermal nanotweezers represent a
major breakthrough in nanotechnology. With its unique capabilities and wide
range of potential applications, CRONT is poised to revolutionize various
fields and lead to breakthrough discoveries.
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